From 08c54df850b4ba21671f740f86b792b174bd35b4 Mon Sep 17 00:00:00 2001
From: JoYo <>
Date: Sat, 15 Feb 2020 16:44:22 -0500
Subject: [PATCH] dap and malt extract for the homebrewers

---
 README.mdown | 31 +++++++++++++++++++++++++++----
 1 file changed, 27 insertions(+), 4 deletions(-)

diff --git a/README.mdown b/README.mdown
index f96a61c..116307d 100644
--- a/README.mdown
+++ b/README.mdown
@@ -52,6 +52,7 @@ Eventually you will want to make your own cultures from your harvested mushrooms
 # Sanitize Your Workspace
 
 - [ ] empty spray bottles, chemical resistant
+
 - [ ] star san, sanitizer
 
 The critical time to practice good sanitation is when opening any of your containers and interacting with the spawn medium inside.
@@ -126,9 +127,13 @@ Skipping testing on [agar slants](#agar-slants) works well enough as long as you
 # Culture Mediums
 
 - [ ] food scale, grams
+
 - [ ] distilled water
+
 - [ ] 16 oz wide mouth mason jars with lids
+
 - [ ] electric pressure cooker, stove top, or [autoclave](#autoclave)
+
 - [ ] aluminum foil
 
 imperial | metric
@@ -138,6 +143,7 @@ imperial | metric
 ## Liquid Cultures
 
 - [ ] light corn syrup
+- [ ] diammonium phosphate with urea (optional)
 
 Mixing liquid culture is similar to [agar slants](#agar-slants) without needing to cool the test tubes in any particular position.
 A simple sugar like light corn syrup is dissolved in distilled water to provide mycelium calories for reproduction.
@@ -157,6 +163,8 @@ simple sugar | 15 g | 5 g
 
 3. Add 15 grams of light corn syrup to warm water and stir for 1 minute with a sanitary utensil.
 
+    - Optionally add no more than 1 gram of diammonium phosphate (dap) with urea for yeast nutrients.
+
 4. Cover un-lid jar opening with aluminum foil and [autoclave](#autoclave) for 1 hour.
 
 5. Allow to cool and replace aluminum foil with mason jar lid.
@@ -168,20 +176,23 @@ simple sugar | 15 g | 5 g
 ## Agar Slants
 
 - [ ] malt extract agar
+
 - [ ] 30 to 50 ml test tube with screw cap, [autoclavable](#autoclave)
+
 - [ ] 50 to 100 ml syringe with hollow needle, [autoclavable](#autoclave)
 
 Agar agar is a plant gelatin that solidifies at room temperature, providing a physical surface that mycelium to colonize.
-This is mixed with a simple sugar like malt extract to provide mycelium calories for reproduction.
+When mixed with a simple sugar like malt extract this provides mycelium calories for reproduction.
 Malt Extract Agar (MEA) is available premixed fairly cheap online.
-Mixing 2:3 grams ratio simple sugar to Agar powder is good for saving money in bulk.
+Mixing 2:3 grams ratio of malt extract to agar powder is good for saving money by buying in bulk from a homebrew shop.
 
 Scale the following recipe as needed to fill 1/3 of each test tube or 10 ml:
 
 ingredient | mason jar | ratio
 ---------- | --------- | -----
 water | 300 ml | 100 ml
-MEA | 15 g | 5 g
+agar | 9 g | 3 g
+malt extract | 6 g | 2 g
 
 0. [Sanitize Your Workspace](#sanitize-your-workspace).
 
@@ -191,7 +202,7 @@ MEA | 15 g | 5 g
 
 2. Microwave with a safe cover until boiling.
 
-3. Add 15 grams of MEA to warm water and stir for 1 minute with a sanitary utensil.
+3. Add 9 g of agar and 6 g of malt extract to the warm water and stir well for 1 minute with a sanitary utensil.
 
 4. While the MEA solution is still warm, fill a sanitary syringe with the solution.
 
@@ -248,6 +259,7 @@ This will allow you to walk through the methods without fear of wasting a spore
 ## Cloning
 
 - [ ] paring knife or scalpel
+
 - [ ] 50 to 100 ml syringe with hollow needle, [autoclavable](#autoclave)
 
 Taking mycelium samples from mushroom tissue is called cloning.
@@ -270,6 +282,7 @@ Piercing the mushroom tissue with a hollow needled syringe is enough to collect
 ## Spore Samples
 
 - [ ] inoculation loop
+
 - [ ] flame or lighter
 
 [Spore](https://en.wikipedia.org/wiki/Fungus#Spore_dispersal) samples are created by placing a mushroom cap on a flat surface and collecting the spores that are released.
@@ -354,11 +367,17 @@ I leave it up to you to research your mycelium species.
 # Grain Spawn
 
 - [ ] 16 oz wide mouth mason jars with lids
+
 - [ ] wild bird seed with millet
+
 - [ ] colander
+
 - [ ] ladle
+
 - [ ] boiling pot
+
 - [ ] aluminum foil
+
 - [ ] electric pressure cooker, stove top, or [autoclave](#autoclave)
 
 Wild bird seed is dusty and full of bacteria and molds.
@@ -439,7 +458,9 @@ However, exposing more surface area give the mushrooms more room to sprout.
 # Fruiting Chambers
 
 - [ ] medium plastic tubs with lids
+
 - [ ] coconut coir
+
 - [ ] personal fan
 
 Coconut husk (coir) as casing layer and flooring in our fruiting chamber to hold moisture better than the mycelium cake we created in the [grain spawn](#grain-spawn) jar.
@@ -496,6 +517,8 @@ Try to maintain 90% humidity within the fruiting chamber.
 
 - [ ] light corn syrup
 
+- [ ] diammonium phosphate with urea
+
 - [ ] paring knife or scalpel
 
 - [ ] inoculation loop