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general fixes

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JoYo 2020-02-19 23:25:57 -05:00
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1 changed files with 17 additions and 10 deletions
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README.mdown
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@ -166,12 +166,14 @@ simple sugar | 15 g | 5 g
- Optionally add no more than 1 gram of diammonium phosphate (dap) with urea for yeast nutrients.
4. Cover un-lid jar opening with aluminum foil and [autoclave](#autoclave) for 1 hour.
Allow to cool to room temperature.
5. Allow to cool and replace aluminum foil with mason jar lid, screwing closed tightly to prevent spilling.
5. Replace aluminum foil with mason jar lid, screwing closed tightly to prevent spilling.
6. Store in a dark cool place until inoculation.
7. Once inoculated, shake the mason jars daily to oxygenate the water and break up the mycelium.
7. Daily shake the mason jars daily to release carbon dioxide and oxygenate the water.
Unscrew the cap and slightly lift the lid to allow the carbon dioxide to escape.
## Agar Slants
@ -271,16 +273,18 @@ Piercing the mushroom tissue with a hollow needled syringe is enough to collect
- Sanitize the outside of each [culture medium](#culture-media).
1. With a sanitary knife, cut the stem off the mushroom.
1. Cut the stem off the mushroom with a sanitary knife, .
2. Pull the plunger of a sanitary syringe to halfway out.
3. Using the hollow needle tip of the syringe, collecting a sample of the core by plunging into the center along the length of the stem.
3. Plunge a hollow needle tip of the syringe into the center along the length of the stem to collect a small sample.
4. Gently use air pressure from the syringe will push the sample into an open [culture medium](#culture-media).
4. Gently push the plunger on the syringe to use air pressure to put the sample into an open [culture medium](#culture-media).
5. Replace the screw cap on the [culture medium](#culture-media) and store in ambient room light at room temperature.
6. Daily release the pressure within the [culture medium](#culture-media) container by unscrewing the cap.
## Spore Samples
- [ ] inoculation loop
@ -299,9 +303,10 @@ An inoculation loop is used to apply the spore samples to a [culture medium](#cu
- Sanitize the outside of each [culture medium](#culture-media).
1. Using a lighter to heat the loop element of the inoculation loop until it glows red. Allow the loop to cool.
1. Lightly scrape spores off a print delivery medium with a sanitary sharp.
2. Scrape spores off a print delivery medium with a sterile sharp.
2. Heat the loop element of the inoculation loop using a lighter until it glows red.
Allow the loop to cool.
3. Collect spores on the loop by gently rubbing against the loose spores.
@ -309,6 +314,8 @@ An inoculation loop is used to apply the spore samples to a [culture medium](#cu
5. Replace the screw cap on the [culture medium](#culture-media) and store in ambient room light at room temperature.
6. Daily release the pressure within the [culture medium](#culture-media) container by unscrewing the cap.
### Spore Syringe
0. [Sanitize Your Workspace](#sanitize-your-workspace).
@ -416,7 +423,7 @@ imperial | metric
9. Cover un-lid jar opening with aluminum foil and [autoclave](#autoclave) for 2 hours.
10. Carefully screw lid over the foil and jar and allow to cool to room temperature.
10. Lightly screw lid over the foil to secure it in place without sealing the jar and allow to cool to room temperature.
11. Store in a dark cool place until inoculation.
@ -501,7 +508,7 @@ These endophytes do not protect our mushroom roots and will compete for resource
Try to maintain at least 80% humidity within the fruiting chamber.
The water soaked cake and coconut coir should provided more than enough moisture to keep the inside of the clear plastic tubs humid.
It is nessisary to open at least daily to provide fresh air and to clear out the co2 produced by the mycelium.
It is necessary to open at least daily to provide fresh air and to clear out the carbon dioxide produced by the mycelium.
0. If needed, gently mist the mycelium cake and surrounding coconut coir a spray bottle of water until humid.
@ -509,7 +516,7 @@ It is nessisary to open at least daily to provide fresh air and to clear out the
2. Replace the tub lid and check again daily.
Harvest mushrooms after their guills are exposed but before they dump their spores and make a mess.
Harvest mushrooms after their gills are exposed but before they dump their spores and make a mess.
# Checklist