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media medium, put stage descriptions sooner

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JoYo 2020-02-15 23:53:29 -05:00
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@ -27,10 +27,10 @@ It is OK.
Bird seed, agar powder, and sugar water are inexpensive. Bird seed, agar powder, and sugar water are inexpensive.
All other equipment can be reused. All other equipment can be reused.
Saving a [control](https://en.wikipedia.org/wiki/Scientific_control) will help you test for contamination introduced through your technique. Saving a control will help you test for contamination introduced through your technique.
If all fares well you can always end up using it. If all fares well you can always end up using it.
Spawn medium refers to any nutrient rich growing surface, such as grain spawn or culture mediums. Spawn medium refers to any nutrient rich growing surface, such as seed grain or culture media.
# Finding Samples # Finding Samples
@ -49,6 +49,22 @@ When paying good money for a sample try to find liquid culture as they have a pr
Eventually you will want to make your own cultures from your harvested mushrooms. Eventually you will want to make your own cultures from your harvested mushrooms.
# Three Stages
There are three general stages to cultivating mycelium.
1. [Culturing](#culturing), [wiki](https://en.wikipedia.org/wiki/Microbiological_culture)
- Using simple sugar and agar as a surface to test sample mycelium for viability and contamination.
2. [Colonization](#colonization), [wiki](https://en.wikipedia.org/wiki/Colony_(biology)#Microbial_colonies)
- Using grain as food source to promote mycelium growth throughout.
3. [Fruiting](#fruiting), [wiki](https://en.wikipedia.org/wiki/Sporocarp_(fungi))
- Apply the ideal conditions to fully colonized seed grain to promote the growth of mushrooms.
# Sanitize Your Workspace # Sanitize Your Workspace
- [ ] empty spray bottles, chemical resistant - [ ] empty spray bottles, chemical resistant
@ -90,22 +106,6 @@ Slow or hold your breath when inoculating spawn medium to avoid shaking.
Remember that mistakes happen and they look pretty cool when they do. Remember that mistakes happen and they look pretty cool when they do.
# Three Stages
There are three general stages to cultivating mycelium.
1. [Culturing](#culturing), [wiki](https://en.wikipedia.org/wiki/Microbiological_culture)
- Using simple sugar and agar as a surface to test sample mycelium for viability and contamination.
2. [Colonization](#colonization), [wiki](https://en.wikipedia.org/wiki/Colony_(biology)#Microbial_colonies)
- Using grain as food source to promote mycelium growth throughout.
3. [Fruiting](#fruiting), [wiki](https://en.wikipedia.org/wiki/Sporocarp_(fungi))
- Apply the ideal conditions to fully colonized grain spawn to promote the growth of mushrooms.
# Culturing # Culturing
Any samples of mycelium are potentially contaminated. Any samples of mycelium are potentially contaminated.
@ -117,14 +117,14 @@ A week of growth is usually long enough to produce colonies which can be identif
While petri dishes are part of the usual trappings of cultivating microorganisms, they are fairly high maintenance and better suited for a laboratory environment. While petri dishes are part of the usual trappings of cultivating microorganisms, they are fairly high maintenance and better suited for a laboratory environment.
[Agar slants](#agar-slants) provide the same functionality without the need to seal and re-seal with parafilm. [Agar slants](#agar-slants) provide the same functionality without the need to seal and re-seal with parafilm.
The preferred [culture medium](#culture-mediums) is [liquid cultures](#liquid-cultures). The preferred [culture medium](#culture-media) is [liquid cultures](#liquid-cultures).
[Liquid cultures](#liquid-cultures) provide a similar micro climate as [agar slants](#agar-slants) with the added benefit of being a much easier for inoculating [grain spawn](#grain-spawn). [Liquid cultures](#liquid-cultures) provide a similar micro climate as [agar slants](#agar-slants) with the added benefit of being a much easier for inoculating [grain spawn](#grain-spawn).
The disadvantage of liquid culture is they are a little difficult to identify contaminations. The disadvantage of liquid culture is they are a little difficult to identify contaminations.
You may use [agar slants](#agar-slants) to isolate mycelium from contamination before transferring to [liquid cultures](#liquid-cultures). You may use [agar slants](#agar-slants) to isolate mycelium from contamination before transferring to [liquid cultures](#liquid-cultures).
Skipping testing on [agar slants](#agar-slants) works well enough as long as you give [liquid cultures](#liquid-cultures) extra time for bacteria to become visible while suspended in water. Skipping testing on [agar slants](#agar-slants) works well enough as long as you give [liquid cultures](#liquid-cultures) extra time for bacteria to become visible while suspended in water.
# Culture Mediums # Culture Media
- [ ] food scale, grams - [ ] food scale, grams
@ -252,7 +252,7 @@ Use the following durations as they make sense to sanitize and pasteurize.
# Culture Inoculation # Culture Inoculation
Once you autoclave your [culture mediums](#culture-mediums) and they have cooled to room temperature it is ready for mycelium. Once you autoclave your [culture media](#culture-media) and they have cooled to room temperature it is ready for mycelium.
Depending on the source of the sample there are two ways to inoculate, [cloning](#cloning) from a mushroom or using [spore samples](spore-samples). Depending on the source of the sample there are two ways to inoculate, [cloning](#cloning) from a mushroom or using [spore samples](spore-samples).
Newbies should start with [cloning](#cloning) grocery store mushrooms. Newbies should start with [cloning](#cloning) grocery store mushrooms.
@ -269,7 +269,7 @@ Piercing the mushroom tissue with a hollow needled syringe is enough to collect
0. [Sanitize Your Workspace](#sanitize-your-workspace). 0. [Sanitize Your Workspace](#sanitize-your-workspace).
- Sanitize the outside of each [culture medium](#culture-mediums). - Sanitize the outside of each [culture medium](#culture-media).
1. With a sanitary knife, cut the stem off the mushroom. 1. With a sanitary knife, cut the stem off the mushroom.
@ -277,9 +277,9 @@ Piercing the mushroom tissue with a hollow needled syringe is enough to collect
3. Using the hollow needle tip of the syringe, collecting a sample of the core by plunging into the center along the length of the stem. 3. Using the hollow needle tip of the syringe, collecting a sample of the core by plunging into the center along the length of the stem.
4. Gently use air pressure from the syringe will push the sample into an open [culture medium](#culture-mediums). 4. Gently use air pressure from the syringe will push the sample into an open [culture medium](#culture-media).
5. Replace the screw cap on the [culture medium](#culture-mediums) and store in ambient room light at room temperature. 5. Replace the screw cap on the [culture medium](#culture-media) and store in ambient room light at room temperature.
## Spore Samples ## Spore Samples
@ -291,13 +291,13 @@ Piercing the mushroom tissue with a hollow needled syringe is enough to collect
These are called spore prints. These are called spore prints.
Spores can then be added to a syringe of sterile water for ease in shipping. Spores can then be added to a syringe of sterile water for ease in shipping.
An inoculation loop is used to apply the spore samples to a [culture medium](#culture-mediums). An inoculation loop is used to apply the spore samples to a [culture medium](#culture-media).
### Spore Print ### Spore Print
0. [Sanitize Your Workspace](#sanitize-your-workspace). 0. [Sanitize Your Workspace](#sanitize-your-workspace).
- Sanitize the outside of each [culture medium](#culture-mediums). - Sanitize the outside of each [culture medium](#culture-media).
1. Using a lighter to heat the loop element of the inoculation loop until it glows red. Allow the loop to cool. 1. Using a lighter to heat the loop element of the inoculation loop until it glows red. Allow the loop to cool.
@ -305,23 +305,23 @@ An inoculation loop is used to apply the spore samples to a [culture medium](#cu
3. Collect spores on the loop by gently rubbing against the loose spores. 3. Collect spores on the loop by gently rubbing against the loose spores.
4. Insert loop into an open [culture medium](#culture-mediums) and gently apply spores. 4. Insert loop into an open [culture medium](#culture-media) and gently apply spores.
5. Replace the screw cap on the [culture medium](#culture-mediums) and store in ambient room light at room temperature. 5. Replace the screw cap on the [culture medium](#culture-media) and store in ambient room light at room temperature.
### Spore Syringe ### Spore Syringe
0. [Sanitize Your Workspace](#sanitize-your-workspace). 0. [Sanitize Your Workspace](#sanitize-your-workspace).
- Sanitize the outside of each [culture medium](#culture-mediums). - Sanitize the outside of each [culture medium](#culture-media).
1. Using a lighter, heat the loop element of your inoculation loop until it glows red. Allow the loop to cool. 1. Using a lighter, heat the loop element of your inoculation loop until it glows red. Allow the loop to cool.
2. Place a single drop of water from a spore syringe on the loop. 2. Place a single drop of water from a spore syringe on the loop.
3. Insert loop into an open [culture medium](#culture-mediums) and gently apply spores. 3. Insert loop into an open [culture medium](#culture-media) and gently apply spores.
4. Replace the screw cap on the [culture medium](#culture-mediums) and store in ambient room light at room temperature. 4. Replace the screw cap on the [culture medium](#culture-media) and store in ambient room light at room temperature.
## Wood Plugs ## Wood Plugs
@ -346,7 +346,7 @@ Otherwise, a successful agar slant colony is used to create [liquid culture](#li
0. [Sanitize Your Workspace](#sanitize-your-workspace). 0. [Sanitize Your Workspace](#sanitize-your-workspace).
- Sanitize the outside of each [culture medium](#culture-mediums). - Sanitize the outside of each [culture medium](#culture-media).
1. Using a lighter, heat the loop element of your inoculation loop until it glows red. Allow the loop to cool. 1. Using a lighter, heat the loop element of your inoculation loop until it glows red. Allow the loop to cool.