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JoYo 2020-02-29 15:18:53 -05:00
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@ -167,7 +167,7 @@ simple sugar | 15 g | 5 g
- Optionally add no more than 1 gram of diammonium phosphate (dap) with urea for yeast nutrients. - Optionally add no more than 1 gram of diammonium phosphate (dap) with urea for yeast nutrients.
4. Cover un-lid jar opening with aluminum foil and [autoclave](#autoclave) for 10 minutes. 4. Cover jar opening with aluminum foil and [autoclave](#autoclave) for 20 minutes.
Allow to cool to room temperature. Allow to cool to room temperature.
5. Replace aluminum foil with mason jar lid, screwing closed tightly to prevent spilling. 5. Replace aluminum foil with mason jar lid, screwing closed tightly to prevent spilling.
@ -214,7 +214,7 @@ malt extract | 6 g | 2 g
5. Fill a sanitary test tube one third (1/3) capacity and replace the screw cap. 5. Fill a sanitary test tube one third (1/3) capacity and replace the screw cap.
6. Place test tubes in an empty mason jar and [autoclave](#autoclave) for 10 minutes. 6. Place test tubes in an empty mason jar and [autoclave](#autoclave) for 20 minutes.
7. Carefully remove each test tube and place on a flat surface with the screw cap slightly elevated on a dish towel. 7. Carefully remove each test tube and place on a flat surface with the screw cap slightly elevated on a dish towel.
@ -365,10 +365,17 @@ Wood-loving species like oyster and shiitake thrive in a mixture of sawdust.
This guide does not address these special requirements. This guide does not address these special requirements.
I leave it up to you to research your mycelium species. I leave it up to you to research your mycelium species.
[Grain spawn](#grain-spawn) is a common source of nutrients and the most accessible source of grain spawn is wild bird seed. Mushroom growers have had some success with the much quicker method of inoculating store bought sterilized whole brown rice.
You should, however, provide mycelium a variety of [grain spawn](#grain-spawn) and [substrates](#bulk-substrate) between generations of colonies so the strain does not lose it's ability to digest different sources of nutrition. These are usually sold as
These general instructions are the same for most [grain spawn](#grain-spawn). > Instant Microwavable Whole Grain Brown Rice
For your first time I recommend you try this method to learn the process.
Simply cut a small hole in a corner of the sterile container and follow the instructions for [spawn inoculation](#spawn-inoculation).
Tape the small hole closed.
You should, however, provide mycelium a variety of [grain spawn](#grain-spawn) and [substrates](#bulk-substrate) between generations of colonies so the strain does not lose it's ability to digest different sources of nutrition.
These general instructions are the same for most [grain spawn](#grain-spawn) but the most accessible source of grain spawn is `wild bird seed`.
# Grain Spawn # Grain Spawn
@ -415,7 +422,7 @@ Imperial | Metric
8. Split evenly to each 16 oz wide mouth mason jar, about 1 cup. 8. Split evenly to each 16 oz wide mouth mason jar, about 1 cup.
9. Cover un-lid jar opening with aluminum foil and [autoclave](#autoclave) for 2 hours. 9. Cover jar opening with aluminum foil and [autoclave](#autoclave) for 2 hours.
10. Lightly screw lid over the foil to secure it in place without sealing the jar and allow to cool to room temperature. 10. Lightly screw lid over the foil to secure it in place without sealing the jar and allow to cool to room temperature.
@ -468,6 +475,9 @@ However, exposing more surface area give the mushrooms more room to sprout.
- [ ] take-out containers, black opaque, polypropylene - [ ] take-out containers, black opaque, polypropylene
Earlier it was mentioned that mycelium want a variety of [grain spawn](#grain-spawn) and [substrates](https://en.wikipedia.org/wiki/Substrate_(biology)) between generations of colonies so the strain does not lose it's ability to digest different sources of nutrition. Earlier it was mentioned that mycelium want a variety of [grain spawn](#grain-spawn) and [substrates](https://en.wikipedia.org/wiki/Substrate_(biology)) between generations of colonies so the strain does not lose it's ability to digest different sources of nutrition.
Once you have practice making [grain spawn](#grain-spawn) you'll want to mix 25% of the substrate chosen for this step to give the mycelium time to produce the right enzymes during colonization.
This guide uses coconut husk (coir) mixed with [grain spawn](#grain-spawn) for colonization as a substrate. This guide uses coconut husk (coir) mixed with [grain spawn](#grain-spawn) for colonization as a substrate.
You can also add coffee grounds, sawdust, straw, and rice husks for variety. You can also add coffee grounds, sawdust, straw, and rice husks for variety.
Take caution to only change one thing at a time between generations or you risk overwhelming the mycelium. Take caution to only change one thing at a time between generations or you risk overwhelming the mycelium.