diff --git a/README.mdown b/README.mdown index 9a1268e..15a7f73 100644 --- a/README.mdown +++ b/README.mdown @@ -82,7 +82,7 @@ If you are working near your living space then a food safe sanitizer like [Star It is very concentrate, a couple drops into a spray bottle full of water is enough. You will know if the solution is diluted enough when it sprays easily and creates small bubbles on the sprayed surface. -It is important to note that sanitization is not sterile. +It is important to note that sanitation is not sterile. Star San does not kill all bacteria or mold. There is always a risk of contamination. @@ -116,7 +116,7 @@ A small culture will allow you to grow a test batch of your sample without for a The preferred culture medium is [liquid cultures](#liquid-cultures). [Liquid cultures](#liquid-cultures) provide a similar micro climate as agar slants with the added benefit of being a much easier for inoculating [grain spawn](#grain-spawn-and-substrate). -The disadvantage of liquid culture is they are a little difficult to identify contaminations. +The disadvantage of liquid culture is they are a little difficult to identify contamination. You may use agar slants to isolate mycelium from contamination before transferring to [liquid cultures](#liquid-cultures). This guide does not cover their use. @@ -177,13 +177,13 @@ simple sugar | 15 g | 5 g ## Autoclave -[Autoclave](https://en.wikipedia.org/wiki/Autoclave) is a generic term for pressurized steam chambers for sanitization and sterilization. +[Autoclave](https://en.wikipedia.org/wiki/Autoclave) is a generic term for pressurized steam chambers for sanitation and sterilization. **All autoclave duration measurements use electric pressure cookers for convenience.** If you have a stove top pressure cooker or industrial autoclave that provide a continuous 100 kPa (15 psi), you may reduce the time by 1/3 for grain spawn. -Autoclavable tools are safe to bring to 120 celsius for extended durations without damage. +Autoclavable tools are safe to bring to 120 celsius for extended duration without damage. 0. Add the steam rack to the inner pot of the electric pressure cooker.