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variety in substrate

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JoYo 2020-02-28 18:15:51 -05:00
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@ -24,7 +24,7 @@ As a result of the above hubris we triple our efforts.
- Make one extra of the above as a control for testing your sanitation. - Make one extra of the above as a control for testing your sanitation.
It is OK. It is OK.
Bird seed, agar powder, and sugar water are inexpensive. Bird seed, coconut husk, and sugar water are inexpensive.
All other equipment can be reused. All other equipment can be reused.
Saving a control will help you test for contamination introduced through your technique. Saving a control will help you test for contamination introduced through your technique.
@ -136,7 +136,7 @@ Skipping testing on [agar slants](#agar-slants) works well enough as long as you
- [ ] aluminum foil - [ ] aluminum foil
imperial | metric Imperial | Metric
-------- | ------ -------- | ------
16 oz | 450 ml 16 oz | 450 ml
@ -148,7 +148,7 @@ imperial | metric
Mixing liquid culture is similar to [agar slants](#agar-slants) without needing to cool the test tubes in any particular position. Mixing liquid culture is similar to [agar slants](#agar-slants) without needing to cool the test tubes in any particular position.
A simple sugar like light corn syrup is dissolved in distilled water to provide mycelium calories for reproduction. A simple sugar like light corn syrup is dissolved in distilled water to provide mycelium calories for reproduction.
ingredient | mason jar | ratio Ingredient | Mason Jar | Ratio
---------- | --------- | ----- ---------- | --------- | -----
water | 300 ml | 100 ml water | 300 ml | 100 ml
simple sugar | 15 g | 5 g simple sugar | 15 g | 5 g
@ -192,7 +192,7 @@ Mixing 2:3 grams ratio of malt extract to agar powder is good for saving money b
Scale the following recipe as needed to fill 1/3 of each test tube or 10 ml: Scale the following recipe as needed to fill 1/3 of each test tube or 10 ml:
ingredient | mason jar | ratio Ingredient | Mason Jar | Ratio
---------- | --------- | ----- ---------- | --------- | -----
water | 300 ml | 100 ml water | 300 ml | 100 ml
agar | 9 g | 3 g agar | 9 g | 3 g
@ -372,9 +372,9 @@ This guide does not address these special requirements.
I leave it up to you to research your mycelium species. I leave it up to you to research your mycelium species.
[Grain spawn](#grain-spawn) is a common source of nutrients and the most accessible source of grain spawn is wild bird seed. [Grain spawn](#grain-spawn) is a common source of nutrients and the most accessible source of grain spawn is wild bird seed.
You should, however, provide mycelium a variety of grain spawn and [substrates](https://en.wikipedia.org/wiki/Substrate_(biology)) between generations of colonies so the strain does not lose it's ability to digest different sources of nutrition. You should, however, provide mycelium a variety of [grain spawn](#grain-spawn) and [substrates](#bulk-substrate) between generations of colonies so the strain does not lose it's ability to digest different sources of nutrition.
These general instructions are the same for most grain spawn. These general instructions are the same for most [grain spawn](#grain-spawn).
# Grain Spawn # Grain Spawn
@ -396,7 +396,7 @@ Wild bird seed is dusty and full of bacteria and molds.
Because the seeds are dry the bacteria has likely [endosporulated](https://en.wikipedia.org/wiki/Endospore), making it resistant to sanitization. Because the seeds are dry the bacteria has likely [endosporulated](https://en.wikipedia.org/wiki/Endospore), making it resistant to sanitization.
Soaking and boiling the bird seed will clean it up a bit and encourage the endospores to reproduce, leaving them weaker to [autoclave](#autoclave). Soaking and boiling the bird seed will clean it up a bit and encourage the endospores to reproduce, leaving them weaker to [autoclave](#autoclave).
imperial | metric Imperial | Metric
-------- | ------ -------- | ------
16 oz | 450 ml 16 oz | 450 ml
1 cup | 250 ml 1 cup | 250 ml
@ -473,8 +473,10 @@ However, exposing more surface area give the mushrooms more room to sprout.
- [ ] take-out containers, black opaque, polypropylene - [ ] take-out containers, black opaque, polypropylene
Coconut husk (coir) holds moisture better than the mycelium cake we created in the [grain spawn](#grain-spawn) jar. Earlier it was mentioned that mycelium want a variety of [grain spawn](#grain-spawn) and [substrates](https://en.wikipedia.org/wiki/Substrate_(biology)) between generations of colonies so the strain does not lose it's ability to digest different sources of nutrition.
We shall mix it with [grain spawn](#grain-spawn) for colonization as a [substrate](https://en.wikipedia.org/wiki/Substrate_(biology)). This guide uses coconut husk (coir) mixed with [grain spawn](#grain-spawn) for colonization as a substrate.
You can also add coffee grounds, sawdust, straw, and rice husks for variety.
Take caution to only change one thing at a time between generations or you risk overwhelming the mycelium.
Many commercial gardening coir are treated with [endophyte](https://en.wikipedia.org/wiki/Endophyte) to protect plant roots. Many commercial gardening coir are treated with [endophyte](https://en.wikipedia.org/wiki/Endophyte) to protect plant roots.
These endophytes do not protect our mushroom roots and will compete for resources like any other contamination. These endophytes do not protect our mushroom roots and will compete for resources like any other contamination.