variety in substrate
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README.mdown
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README.mdown
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@ -24,7 +24,7 @@ As a result of the above hubris we triple our efforts.
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- Make one extra of the above as a control for testing your sanitation.
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It is OK.
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Bird seed, agar powder, and sugar water are inexpensive.
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Bird seed, coconut husk, and sugar water are inexpensive.
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All other equipment can be reused.
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Saving a control will help you test for contamination introduced through your technique.
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@ -136,7 +136,7 @@ Skipping testing on [agar slants](#agar-slants) works well enough as long as you
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- [ ] aluminum foil
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imperial | metric
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Imperial | Metric
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-------- | ------
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16 oz | 450 ml
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@ -148,7 +148,7 @@ imperial | metric
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Mixing liquid culture is similar to [agar slants](#agar-slants) without needing to cool the test tubes in any particular position.
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A simple sugar like light corn syrup is dissolved in distilled water to provide mycelium calories for reproduction.
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ingredient | mason jar | ratio
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Ingredient | Mason Jar | Ratio
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---------- | --------- | -----
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water | 300 ml | 100 ml
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simple sugar | 15 g | 5 g
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@ -192,7 +192,7 @@ Mixing 2:3 grams ratio of malt extract to agar powder is good for saving money b
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Scale the following recipe as needed to fill 1/3 of each test tube or 10 ml:
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ingredient | mason jar | ratio
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Ingredient | Mason Jar | Ratio
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---------- | --------- | -----
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water | 300 ml | 100 ml
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agar | 9 g | 3 g
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@ -372,9 +372,9 @@ This guide does not address these special requirements.
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I leave it up to you to research your mycelium species.
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[Grain spawn](#grain-spawn) is a common source of nutrients and the most accessible source of grain spawn is wild bird seed.
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You should, however, provide mycelium a variety of grain spawn and [substrates](https://en.wikipedia.org/wiki/Substrate_(biology)) between generations of colonies so the strain does not lose it's ability to digest different sources of nutrition.
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You should, however, provide mycelium a variety of [grain spawn](#grain-spawn) and [substrates](#bulk-substrate) between generations of colonies so the strain does not lose it's ability to digest different sources of nutrition.
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These general instructions are the same for most grain spawn.
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These general instructions are the same for most [grain spawn](#grain-spawn).
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# Grain Spawn
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@ -396,7 +396,7 @@ Wild bird seed is dusty and full of bacteria and molds.
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Because the seeds are dry the bacteria has likely [endosporulated](https://en.wikipedia.org/wiki/Endospore), making it resistant to sanitization.
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Soaking and boiling the bird seed will clean it up a bit and encourage the endospores to reproduce, leaving them weaker to [autoclave](#autoclave).
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imperial | metric
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Imperial | Metric
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-------- | ------
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16 oz | 450 ml
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1 cup | 250 ml
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@ -473,8 +473,10 @@ However, exposing more surface area give the mushrooms more room to sprout.
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- [ ] take-out containers, black opaque, polypropylene
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Coconut husk (coir) holds moisture better than the mycelium cake we created in the [grain spawn](#grain-spawn) jar.
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We shall mix it with [grain spawn](#grain-spawn) for colonization as a [substrate](https://en.wikipedia.org/wiki/Substrate_(biology)).
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Earlier it was mentioned that mycelium want a variety of [grain spawn](#grain-spawn) and [substrates](https://en.wikipedia.org/wiki/Substrate_(biology)) between generations of colonies so the strain does not lose it's ability to digest different sources of nutrition.
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This guide uses coconut husk (coir) mixed with [grain spawn](#grain-spawn) for colonization as a substrate.
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You can also add coffee grounds, sawdust, straw, and rice husks for variety.
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Take caution to only change one thing at a time between generations or you risk overwhelming the mycelium.
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Many commercial gardening coir are treated with [endophyte](https://en.wikipedia.org/wiki/Endophyte) to protect plant roots.
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These endophytes do not protect our mushroom roots and will compete for resources like any other contamination.
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