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JoYo 2020-02-10 18:54:53 -05:00
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@ -213,21 +213,11 @@ Autoclavable tools are safe to bring to 250 celsius for extended durations witho
Use the following durations as they make sense to sanitize and pasteurize.
- 2 hours
- Spawn grain should be autoclave for 2 hours and cooled prior to inoculation.
Bulk grains are particularly susceptible to contamination during the commercial farming process.
- 1 hour
- Cultures do not need to autoclave for as long as spawn grain since there is less volume that needs to get to temperature.
- Add your autoclavable tools if there is space.
If you do not plan to use them soon after autoclave, soak in Star San solution and store covered.
- 10 minutes
- Quickly bring test tubes and tools to temperature for cleaning out old agar or liquid culture.
| | |
-- | --
2 hours | Spawn grain should be autoclave for 2 hours and cooled prior to inoculation. Bulk grains are particularly susceptible to contamination during the commercial farming process.
1 hour | Cultures do not need to autoclave for as long as spawn grain since there is less volume that needs to get to temperature. Add your autoclavable tools if there is space. If you do not plan to use them soon after autoclave, soak in Star San solution and store covered.
10 minutes | Quickly bring test tubes and tools to temperature for cleaning out old agar or liquid culture.
0. Add the steam rack to the inner pot of the electric pressure cooker.