diff --git a/README.mdown b/README.mdown index 1461a55..9dd717d 100644 --- a/README.mdown +++ b/README.mdown @@ -170,7 +170,7 @@ simple sugar | 15 g | 5 g - Optionally add no more than 1 gram of diammonium phosphate (dap) with urea for yeast nutrients. -4. Cover jar opening with aluminum foil and [autoclave](#autoclave) for 20 minutes. +4. Cover jar opening with aluminum foil and [autoclave](#autoclave) for 10 minutes. Allow to cool to room temperature. 5. Replace aluminum foil with mason jar lid, screwing closed tightly to prevent spilling. @@ -215,7 +215,7 @@ light corn syrup | 6 g | 2 g 5. Fill a sanitary test tube one third (1/3) capacity and replace the screw cap. -6. Place test tubes in an empty mason jar and [autoclave](#autoclave) for 20 minutes. +6. Place test tubes in an empty mason jar and [autoclave](#autoclave) for 10 minutes. 7. Carefully remove each test tube and place on a flat surface with the screw cap slightly elevated on a dish towel. @@ -230,7 +230,7 @@ light corn syrup | 6 g | 2 g **All autoclave duration measurements use electric pressure cookers for convenience.** -If you have a stove top pressure cooker or industrial autoclave that provide a continuous 100 kPa (15 psi), you may reduce the time by 1/3. +If you have a stove top pressure cooker or industrial autoclave that provide a continuous 100 kPa (15 psi), you may reduce the time by 1/3 for grain spawn. Autoclavable tools are safe to bring to 120 celsius for extended durations without damage.