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README.mdown
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README.mdown
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@ -311,19 +311,18 @@ Mycelium samples are often sold as colonized grain or wood plugs.
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Usually these can be used to inoculate [grain spawn](#grain-spawn) directly.
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Cultures are used to store these samples long-term.
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With sanitary tweezers place a sample in an [agar slant](#agar-slants), [liquid culture](#liquid-cultures), or grain spawn.
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Use sanitary tweezers to place a sample in spawn medium.
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# Reading an Agar Slant
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[Agar slants](#agar-slants) should show growth within the first week and should be monitored for contamination weekly.
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[Agar slants](#agar-slants) should show growth within the first week after inoculation and should be monitored for contamination weekly.
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Mycelium are snowy white with grey and blue tints as normal.
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Any other colors and you are dealing with contamination.
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Contamination is not the end of the world.
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Mycelium can be isolated from a contaminated sample.
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If enough mycelium growth has occurred that a sample can be collected without touching the contamination then it can be transferred to a fresh [culture medium](#culture-mediums).
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If enough mycelium growth has occurred that a sample can be collected without touching the contamination then it can be transferred to fresh [agar slants](#agar-slants) and tested again.
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Otherwise, a successful agar slant colony is used to create [liquid culture](#liquid-cultures).
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0. [Sanitize Your Workspace](#sanitize-your-workspace).
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@ -331,15 +330,15 @@ If enough mycelium growth has occurred that a sample can be collected without to
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1. Using a lighter, heat the loop element of your inoculation loop until it glows red. Allow the loop to cool.
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2. Gently scrape some of the mycelium onto the loop.
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2. Gently scrape some of the mycelium off of the surface of agar onto the loop.
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3. Insert loop into an open [culture medium](#culture-mediums) and gently apply spores.
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3. Insert loop into an open [liquid culture](#liquid-cultures) and gently apply spores.
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4. Replace the screw cap on the [culture medium](#culture-mediums) and store in ambient room light at room temperature.
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4. Replace the screw cap on the [liquid culture](#liquid-cultures) and store in ambient room light at room temperature.
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# Colonization
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Once the mycelium has been successfully isolated and grown in [liquid cultures](#liquid-cultures) the mycelium wants something a bit more nutritious than sugar water.
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Once the mycelium is successfully isolated in [liquid cultures](#liquid-cultures), the mycelium wants something a bit more nutritious than sugar water.
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Most species have special requirements that encourage the growth of mushrooms.
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Wood-loving species like oyster and shiitake thrive in a mixture of wood chips.
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This guide does not address these special requirements.
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@ -357,13 +356,18 @@ I leave it up to you to research your mycelium species.
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- [ ] aluminum foil
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- [ ] electric pressure cooker, stove top, or [autoclave](#autoclave)
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Wild bird seed is rather dusty and full of bacteria and molds.
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Because the seeds are dry the bacteria has likely [endosporulated](https://en.wikipedia.org/wiki/Endospore) making it resistant to heat.
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Soaking and boiling the bird seed will clean it up a bit and encourage the endospores to reproduce, leaving them weaker to autoclave.
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Wild bird seed is dusty and full of bacteria and molds.
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Because the seeds are dry the bacteria has likely [endosporulated](https://en.wikipedia.org/wiki/Endospore), making it resistant to sanitization.
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Soaking and boiling the bird seed will clean it up a bit and encourage the endospores to reproduce, leaving them weaker to [autoclave](#autoclave).
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imperial | metric
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-------- | ------
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16 oz | 450 ml
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1 cup | 250 ml
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0. [Sanitize Your Workspace](#sanitize-your-workspace).
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1. Fill 16 oz wide mouth mason jars with 1 cup of bird seed per final jar of grain spawn.
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1. Fill 16 oz wide mouth mason jars with 1 cup of bird seed for each final jar of grain spawn.
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2. Cover bird seed with tap water, lid jar, and soak for 12 to 24 hours.
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@ -405,7 +409,7 @@ Soaking and boiling the bird seed will clean it up a bit and encourage the endos
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# Reading a Spawn Jar
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Similar to [Reading an Agar Slant](#reading-an-agar-slant), [grain spawn](#grain-spawn) should show growth within the first week and should be monitored for contamination weekly until it is fully colonized, 2-3 weeks.
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Similar to [Reading an Agar Slant](#reading-an-agar-slant), [grain spawn](#grain-spawn) should show growth within the first week after inoculation and should be monitored for contamination weekly until it is fully colonized, 2-3 weeks.
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Mycelium are snowy white with grey and blue tints as normal.
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@ -421,9 +425,9 @@ This is because mycelium sense that water is evaporating and wish to take advant
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In our fruiting containers we want to mimic that as much as possible with a spray bottle and a light breeze.
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This can be accomplished with an open and fully colonized [grain spawn](#grain-spawn) jar and a daily spray bottle misting.
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This can be accomplished by opening a fully colonized [grain spawn](#grain-spawn) jar and misting the mycelium cake with water daily.
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However, if there is more surface area for the mushroom pins to sprout from then there is a higher chance of successful fruiting.
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However, exposing more surface area give the mushrooms more room to sprout.
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# Fruiting Chamber
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@ -433,9 +437,9 @@ However, if there is more surface area for the mushroom pins to sprout from then
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Coconut husk (coir) as casing layer and flooring in our fruiting chamber to hold moisture better than the mycelium cake we created in the [grain spawn](#grain-spawn) jar.
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0. Invert an open and fully colonized [grain spawn](#grain-spawn) jar and firmly but gently tap cake out.
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0. Open and invert a fully colonized [grain spawn](#grain-spawn) jar and firmly but gently tap the mycelium cake out.
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- If the cake breaks up that is OK, place the pieces back together if possible.
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- If the cake breaks up that is OK, place the pieces back together when they are rolled in coir.
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1. Soak the mycelium cake in water for 12 to 24 hours.
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@ -443,7 +447,7 @@ Coconut husk (coir) as casing layer and flooring in our fruiting chamber to hold
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3. Gently roll the mycelium cake in the hydrated coconut coir to form a light casing.
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4. Layer the bottom of the tub with an inch or more of coconut coir.
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4. Layer the bottom of the tub with 5 cm or more of coconut coir.
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5. Place the mycelium cake on the layer of coconut coir away from the sides.
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@ -451,11 +455,11 @@ Coconut husk (coir) as casing layer and flooring in our fruiting chamber to hold
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Try to maintain 90% humidity within the fruiting chamber.
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0. With a water spray bottle gently mist the coconut coir and mycelium cake until the coir is moist but not soaked.
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0. With a spray bottle of water, gently mist the coconut coir and mycelium cake until the coir is moist but not soaked.
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1. Place the medium plastic tub lid upside down, slightly ajar, and rest for 1 hour.
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1. Place the medium plastic tub lid upside down on the tub slightly ajar and rest for 1 hour.
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2. Point a personal fan nearby to blow towards the ceiling above the tub for 1 hour.
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2. Place a personal fan nearby to blow upwards towards the ceiling above the tub for 1 hour.
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3. Replace the lid upside down and slightly ajar.
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