diff --git a/README.mdown b/README.mdown index b01451f..4138c1e 100644 --- a/README.mdown +++ b/README.mdown @@ -167,7 +167,7 @@ simple sugar | 15 g | 5 g - Optionally add no more than 1 gram of diammonium phosphate (dap) with urea for yeast nutrients. -4. Cover un-lid jar opening with aluminum foil and [autoclave](#autoclave) for 1 hour. +4. Cover un-lid jar opening with aluminum foil and [autoclave](#autoclave) for 10 minutes. Allow to cool to room temperature. 5. Replace aluminum foil with mason jar lid, screwing closed tightly to prevent spilling. @@ -214,7 +214,7 @@ malt extract | 6 g | 2 g 5. Fill a sanitary test tube one third (1/3) capacity and replace the screw cap. -6. Place test tubes in an empty mason jar and [autoclave](#autoclave) for 1 hour. +6. Place test tubes in an empty mason jar and [autoclave](#autoclave) for 10 minutes. 7. Carefully remove each test tube and place on a flat surface with the screw cap slightly elevated on a dish towel. @@ -233,14 +233,6 @@ If you have a stove top pressure cooker or industrial autoclave that provide a c Autoclavable tools are safe to bring to 120 celsius for extended durations without damage. -Use the following durations as they make sense to sanitize and pasteurize. - -| | | --- | -- -10 minutes | Quickly bring test tubes and tools to temperature for cleaning out old agar or liquid culture. -1 hour | Cultures have less volume to get to temperature. Add your autoclavable tools if there is space. -2 hours | Bulk grains are particularly susceptible to contamination during the commercial farming process. - 0. Add the steam rack to the inner pot of the electric pressure cooker. 1. Add 200 ml of water to the inner pot.