removing unessisary utensils
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README.mdown
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README.mdown
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@ -280,18 +280,29 @@ Piercing the mushroom tissue with a hollow needled syringe is enough to collect
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## Spore Samples
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## Spore Samples
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- [ ] inoculation loop
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- [ ] flame or lighter
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- [ ] flame or lighter
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[Spore](https://en.wikipedia.org/wiki/Fungus#Spore_dispersal) samples are created by placing a mushroom cap on a flat surface and collecting the spores that are released.
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[Spore](https://en.wikipedia.org/wiki/Fungus#Spore_dispersal) samples are created by placing a mushroom cap on a flat surface and collecting the spores that are released.
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These are called spore prints.
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These are called spore prints.
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Spores can then be added to a syringe of sterile water for ease in shipping.
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Spores can then be added to a syringe of sterile water for ease in shipping.
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An inoculation loop is used to apply the spore samples to a [culture medium](#culture-media).
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### Spore Syringe
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0. [Sanitize Your Workspace](#sanitize-your-workspace).
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- Sanitize the outside of each [culture medium](#culture-media).
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1. Using a lighter, heat the spore syringe needle until it glows red. Allow to cool.
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2. Place a single drop of water from a spore syringe on the [culture medium](#culture-media).
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3. Replace the screw cap on the [culture medium](#culture-media) and store in ambient room light at room temperature.
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### Spore Print
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### Spore Print
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An inoculation loop maybe useful to apply the spore print samples to a [culture medium](#culture-media).
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Alternatively use a sanitary sharp knife or scalpel to apply the spore print samples.
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0. [Sanitize Your Workspace](#sanitize-your-workspace).
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0. [Sanitize Your Workspace](#sanitize-your-workspace).
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- Sanitize the outside of each [culture medium](#culture-media).
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- Sanitize the outside of each [culture medium](#culture-media).
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@ -309,24 +320,8 @@ An inoculation loop is used to apply the spore samples to a [culture medium](#cu
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6. Daily release the pressure within the [culture medium](#culture-media) container by unscrewing the cap.
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6. Daily release the pressure within the [culture medium](#culture-media) container by unscrewing the cap.
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### Spore Syringe
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0. [Sanitize Your Workspace](#sanitize-your-workspace).
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- Sanitize the outside of each [culture medium](#culture-media).
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1. Using a lighter, heat the loop element of your inoculation loop until it glows red. Allow the loop to cool.
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2. Place a single drop of water from a spore syringe on the loop.
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3. Insert loop into an open [culture medium](#culture-media) and gently apply spores.
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4. Replace the screw cap on the [culture medium](#culture-media) and store in ambient room light at room temperature.
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## Wood Plugs
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## Wood Plugs
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- [ ] tweezers
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Mycelium samples are often sold as colonized grain or wood plugs.
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Mycelium samples are often sold as colonized grain or wood plugs.
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Usually these can be used to inoculate [grain spawn](#grain-spawn) directly.
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Usually these can be used to inoculate [grain spawn](#grain-spawn) directly.
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Cultures are used to store these samples long-term.
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Cultures are used to store these samples long-term.
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@ -348,11 +343,11 @@ Otherwise, a successful agar slant colony is used to create [liquid culture](#li
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- Sanitize the outside of each [culture medium](#culture-media).
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- Sanitize the outside of each [culture medium](#culture-media).
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1. Using a lighter, heat the loop element of your inoculation loop until it glows red. Allow the loop to cool.
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1. Using a lighter, heat a sharp knife or scalpel until it glows red. Allow to cool.
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2. Gently scrape some of the mycelium off of the surface of agar onto the loop.
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2. Carefully cut a small sample of the mycelium colonized agar off.
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3. Insert loop into an open [liquid culture](#liquid-cultures) and gently apply spores.
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3. Insert agar sample into an open [liquid culture](#liquid-cultures) to apply spores.
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4. Replace the screw cap on the [liquid culture](#liquid-cultures) and store in ambient room light at room temperature.
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4. Replace the screw cap on the [liquid culture](#liquid-cultures) and store in ambient room light at room temperature.
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@ -557,12 +552,8 @@ Harvest mushrooms after their gills are exposed but before they dump their spore
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- [ ] 30 to 50 ml test tubes with screw cap, [autoclavable](#autoclave)
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- [ ] 30 to 50 ml test tubes with screw cap, [autoclavable](#autoclave)
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- [ ] inoculation loop
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- [ ] flame or lighter
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- [ ] flame or lighter
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- [ ] tweezers
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- [ ] wild bird seed with millet
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- [ ] wild bird seed with millet
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- [ ] colander
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- [ ] colander
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