removed more optional steps, simplified instructions
JoYo
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5fd4309ef8
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d877c32912
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README.mdown
299
README.mdown
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@ -11,26 +11,24 @@ Contamination occurs when other microorganisms take advantage of the gratuity yo
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There is always a risk that bacteria and mold will invade, competing with your precious mushroom makers.
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Many guides do everything they can to reduce this risk.
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My approach gives you methods that are still effective but easy and cheap to recover from contaminated spawn.
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My approach gives you methods that are still effective but easy and cheap to recover from contamination.
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# Do It Thrice
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As a result of the above hubris we triple our efforts.
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- Make at least two agar slants or liquid cultures for each sample we wish to test.
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- Make at least two liquid cultures for each sample we wish to test.
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- Make at least two colonization jars for each sample we wish to colonize.
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- Make one extra of the above as a control for testing your sanitation.
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- Make one extra liquid culture and colonization jar as a control for testing your sanitation.
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It is OK.
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Bird seed, coconut husk, and sugar water are inexpensive.
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All other equipment can be reused.
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Saving a control will help you test for contamination introduced through your technique.
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If all fares well you can always end up using it.
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Spawn medium refers to any nutrient rich growing surface, such as seed grain or culture media.
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`Spawn medium` refers to any nutrient rich growing surface, such as seed grain or simple sugar.
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# Finding Samples
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@ -53,9 +51,9 @@ Eventually you will want to make your own cultures from your harvested mushrooms
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There are three general stages to cultivating mycelium.
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1. [Culturing](#culturing), [wiki](https://en.wikipedia.org/wiki/Microbiological_culture)
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1. [Culturing](#mycelium-cultures), [wiki](https://en.wikipedia.org/wiki/Microbiological_culture)
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- Using simple sugar and agar as a surface to test sample mycelium for viability and contamination.
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- Using simple sugar solution to test a sample of mycelium for viability and contamination.
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2. [Colonization](#colonization), [wiki](https://en.wikipedia.org/wiki/Colony_(biology)#Microbial_colonies)
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@ -108,25 +106,23 @@ Slow or hold your breath when inoculating spawn medium to avoid shaking.
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Remember that mistakes happen and they look pretty cool when they do.
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# Culturing
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# Mycelium Cultures
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Any samples of mycelium are potentially contaminated.
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Even using a microscope and visually checking the entire sample is tedious, error prone, and impractical.
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A small culture will allow you to grow a test batch of your sample without for a jar of [grain spawn](#grain-spawn) to show signs of contamination.
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A small culture will allow you to grow a test batch of your sample without for a jar of [grain spawn](#grain-spawn-and-substrate) to show signs of contamination.
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Agar gives mycelium and contamination a surface to grow in large enough colonies to view with the naked eye.
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A week of growth is usually long enough to produce colonies which can be identified and transferred to [liquid cultures](#liquid-cultures).
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While petri dishes are part of the usual trappings of cultivating microorganisms, they are fairly high maintenance and better suited for a laboratory environment.
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[Agar slants](#agar-slants) provide the same functionality without the need to seal and re-seal with parafilm.
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The preferred [culture medium](#culture-media) is [liquid cultures](#liquid-cultures).
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[Liquid cultures](#liquid-cultures) provide a similar micro climate as [agar slants](#agar-slants) with the added benefit of being a much easier for inoculating [grain spawn](#grain-spawn).
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The preferred culture medium is [liquid cultures](#liquid-cultures).
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[Liquid cultures](#liquid-cultures) provide a similar micro climate as agar slants with the added benefit of being a much easier for inoculating [grain spawn](#grain-spawn-and-substrate).
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The disadvantage of liquid culture is they are a little difficult to identify contaminations.
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You may use [agar slants](#agar-slants) to isolate mycelium from contamination before transferring to [liquid cultures](#liquid-cultures).
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Skipping testing on [agar slants](#agar-slants) works well enough as long as you give [liquid cultures](#liquid-cultures) extra time for bacteria to become visible while suspended in water.
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You may use agar slants to isolate mycelium from contamination before transferring to [liquid cultures](#liquid-cultures).
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This guide does not cover their use.
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Skipping testing on agar slants works well enough as long as you give [liquid cultures](#liquid-cultures) extra time for bacteria to become visible while suspended in water.
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# Culture Media
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# Liquid Cultures
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A simple sugar like light corn syrup is dissolved in distilled water to provide mycelium calories for reproduction.
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- [ ] food scale, grams
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@ -138,7 +134,7 @@ Skipping testing on [agar slants](#agar-slants) works well enough as long as you
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- [ ] light corn syrup
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- [ ] diammonium phosphate with urea (optional)
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- [ ] marbles or magnetic stirring rods
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- [ ] aluminum foil
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@ -148,11 +144,6 @@ Imperial | Metric
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-------- | ------
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16 oz | 450 ml
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## Liquid Cultures
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Mixing liquid culture is similar to [agar slants](#agar-slants) without needing to cool the test tubes in any particular position.
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A simple sugar like light corn syrup is dissolved in distilled water to provide mycelium calories for reproduction.
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Ingredient | Mason Jar | Ratio
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---------- | --------- | -----
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water | 300 ml | 100 ml
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@ -166,64 +157,22 @@ simple sugar | 15 g | 5 g
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2. Microwave with a safe cover until boiling, about 1 minute.
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3. Add 15 grams of light corn syrup to warm water and stir for 1 minute with a sanitary utensil.
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3. Add 15 grams of light corn syrup to warm water.
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- Optionally add no more than 1 gram of diammonium phosphate (dap) with urea for yeast nutrients.
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4. Cover jar opening with aluminum foil and [pressure cook](#autoclave) for 25 minutes at 70 kPa (10 psi).
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4. Insert a marble or magnetic stirring rod for stirring the solution once inncoulated.
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5. Cover jar opening with aluminum foil and [pressure cook](#autoclave) for 25 minutes at 70 kPa (10 psi).
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Allow to cool to room temperature.
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5. Replace aluminum foil with mason jar lid, screwing closed tightly to prevent spilling.
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6. Replace aluminum foil with mason jar lid, screwing closed tightly to prevent spilling.
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6. Store in a dark cool place until inoculation.
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7. Store in a dark cool place until inoculation.
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7. Daily shake the mason jars daily to release carbon dioxide and oxygenate the water.
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8. Daily shake the mason jars daily to release carbon dioxide and oxygenate the water.
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Unscrew the cap and slightly lift the lid to allow the carbon dioxide to escape.
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## Agar Slants
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- [ ] agar
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- [ ] 30 to 50 ml test tubes with screw cap, [autoclavable](#autoclave)
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Agar agar is a plant gelatin that solidifies at room temperature, providing a physical surface that mycelium to colonize.
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When mixed with a simple sugar like light corn syrup this provides mycelium calories for reproduction.
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Malt Extract Agar (MEA) is available premixed fairly cheap online.
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Mixing 2:3 grams ratio of light corn syrup to agar powder is good for saving money by bulk.
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Scale the following recipe as needed to fill 1/3 of each test tube or 10 ml:
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Ingredient | Mason Jar | Ratio
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---------- | --------- | -----
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water | 300 ml | 100 ml
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agar | 9 g | 3 g
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light corn syrup | 6 g | 2 g
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0. [Sanitize Your Workspace](#sanitize-your-workspace).
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1. Fill a 16 oz mason jar with 300 ml water on a food scale.
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- Grams is the same as milliliters of water at [STP](https://en.wikipedia.org/wiki/Standard_conditions_for_temperature_and_pressure).
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2. Microwave with a safe cover until boiling, about 1 minute.
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3. Add 9 g of agar and 6 g of light corn syrup to the warm water and stir well for 1 minute with a sanitary utensil.
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- Optionally add no more than 1 gram of diammonium phosphate (dap) with urea for yeast nutrients.
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4. While the MEA solution is still warm, fill a sanitary syringe with the solution.
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5. Fill a sanitary test tube one third (1/3) capacity and replace the screw cap.
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6. Place test tubes in an empty mason jar and [pressure cook](#autoclave) for 25 minutes at 70 kPa (10 psi).
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7. Carefully remove each test tube and place on a flat surface with the screw cap slightly elevated on a dish towel.
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- Once cooled the agar should solidify into a slanted surface for inoculation.
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8. Store in a dark cool place until inoculation.
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Do not freeze.
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## Autoclave
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[Autoclave](https://en.wikipedia.org/wiki/Autoclave) is a generic term for pressurized steam chambers for sanitization and sterilization.
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@ -240,16 +189,15 @@ Autoclavable tools are safe to bring to 120 celsius for extended durations witho
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2. Insert items to be autoclave on to the steam rack.
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3. Press `Steam` option and set the appropriate time.
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3. Press `Steam` or `Canning` option and set the appropriate time.
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4. Turn vent valve to close.
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5. Once time has completed allow to passively depressurize.
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Venting the valve early may be necessary for working with agar.
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# Culture Inoculation
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Once you autoclave your [culture media](#culture-media) and they have cooled to room temperature it is ready for mycelium.
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Once you autoclave your [liquid cultures](#liquid-cultures) and they have cooled to room temperature it is ready for mycelium.
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Depending on the source of the sample there are two ways to inoculate, [cloning](#cloning) from a mushroom or using [spore samples](spore-samples).
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Newbies should start with [cloning](#cloning) grocery store mushrooms.
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@ -264,7 +212,7 @@ Piercing the mushroom tissue with a hollow needled syringe is enough to collect
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0. [Sanitize Your Workspace](#sanitize-your-workspace).
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- Sanitize the outside of each [culture medium](#culture-media).
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- Sanitize the outside of each [liquid culture](#liquid-cultures).
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1. Cut the stem off the mushroom with a sanitary knife.
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@ -272,11 +220,11 @@ Piercing the mushroom tissue with a hollow needled syringe is enough to collect
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3. Plunge a hollow needle tip of the syringe into the center along the length of the stem to collect a small sample.
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4. Gently push the plunger on the syringe to use air pressure to put the sample into an open [culture medium](#culture-media).
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4. Gently push the plunger on the syringe to use air pressure to put the sample into an open [liquid culture](#liquid-cultures).
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5. Replace the screw cap on the [culture medium](#culture-media) and store in ambient room light at room temperature.
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5. Replace the screw cap on the [liquid culture](#liquid-cultures) and store in ambient room light at room temperature.
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6. Daily release the pressure within the [culture medium](#culture-media) container by unscrewing the cap.
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6. Daily release the pressure within the [liquid culture](#liquid-cultures) container by unscrewing the cap.
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## Spore Samples
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@ -290,22 +238,22 @@ Spores can then be added to a syringe of sterile water for ease in shipping.
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0. [Sanitize Your Workspace](#sanitize-your-workspace).
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- Sanitize the outside of each [culture medium](#culture-media).
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- Sanitize the outside of each [liquid culture](#liquid-cultures).
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1. Using a lighter, heat the spore syringe needle until it glows red. Allow to cool.
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2. Place a single drop of water from a spore syringe on the [culture medium](#culture-media).
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2. Place a single drop of water from a spore syringe on the [liquid culture](#liquid-cultures).
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3. Replace the screw cap on the [culture medium](#culture-media) and store in ambient room light at room temperature.
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3. Replace the screw cap on the [liquid culture](#liquid-cultures) and store in ambient room light at room temperature.
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### Spore Print
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An inoculation loop maybe useful to apply the spore print samples to a [culture medium](#culture-media).
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An inoculation loop maybe useful to apply the spore print samples to a [liquid culture](#liquid-cultures).
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Alternatively use a sanitary sharp knife or scalpel to apply the spore print samples.
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0. [Sanitize Your Workspace](#sanitize-your-workspace).
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- Sanitize the outside of each [culture medium](#culture-media).
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- Sanitize the outside of each [liquid culture](#liquid-cultures).
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1. Lightly scrape spores off a print delivery medium with a sanitary sharp.
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@ -314,42 +262,21 @@ Alternatively use a sanitary sharp knife or scalpel to apply the spore print sam
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3. Collect spores on the loop by gently rubbing against the loose spores.
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4. Insert loop into an open [culture medium](#culture-media) and gently apply spores.
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4. Insert loop into an open [liquid culture](#liquid-cultures) and gently apply spores.
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5. Replace the screw cap on the [culture medium](#culture-media) and store in ambient room light at room temperature.
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5. Replace the screw cap on the [liquid culture](#liquid-cultures) and store in ambient room light at room temperature.
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6. Daily release the pressure within the [culture medium](#culture-media) container by unscrewing the cap.
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6. Daily release the pressure within the [liquid culture](#liquid-cultures) container by unscrewing the cap.
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## Wood Plugs
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# Reading Liquid Cultures
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Mycelium samples are often sold as colonized grain or wood plugs.
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Usually these can be used to inoculate [grain spawn](#grain-spawn) directly.
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Cultures are used to store these samples long-term.
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[Liquid cultures](#liquid-cultures) should show growth within the first week after inoculation and should be monitored for contamination weekly.
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Use sanitary tweezers to place a sample in spawn medium.
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Mycelium are snowy white with grey, blue, or brown tints depending on the species.
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# Reading an Agar Slant
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Colors like yellow, red, green, and black usually indicate contamination.
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[Agar slants](#agar-slants) should show growth within the first week after inoculation and should be monitored for contamination weekly.
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Mycelium are snowy white with grey and blue tints as normal.
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Any other colors and you are dealing with contamination.
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Contamination is not the end of the world.
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If enough mycelium growth has occurred that a sample can be collected without touching the contamination then it can be transferred to fresh [agar slants](#agar-slants) and tested again.
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Otherwise, a successful agar slant colony is used to create [liquid culture](#liquid-cultures).
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0. [Sanitize Your Workspace](#sanitize-your-workspace).
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- Sanitize the outside of each [culture medium](#culture-media).
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1. Using a lighter, heat a sharp knife or scalpel until it glows red. Allow to cool.
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2. Carefully cut a small sample of the mycelium colonized agar off.
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3. Insert agar sample into an open [liquid culture](#liquid-cultures) to apply spores.
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4. Replace the screw cap on the [liquid culture](#liquid-cultures) and store in ambient room light at room temperature.
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Dispose of the contaminated solution being mindful of any stirring rods or marbles you might have inserted.
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# Colonization
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@ -360,7 +287,7 @@ This guide does not address these special requirements.
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I leave it up to you to research your mycelium species.
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Mushroom growers have had some success with the much quicker method of inoculating store bought sterilized whole brown rice.
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These are usually sold as
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These are usually sold as:
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> Instant Microwavable Whole Grain Brown Rice
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@ -368,147 +295,113 @@ For your first time I recommend store bought sterilized whole brown rice to lear
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Simply cut a small hole in a corner of the sterile container and follow the instructions for [spawn inoculation](#spawn-inoculation).
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Tape the small hole closed.
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You should, however, provide mycelium a variety of [grain spawn](#grain-spawn) and [substrates](#bulk-substrate) between generations of colonies so the strain does not lose it's ability to digest different sources of nutrition.
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These general instructions are the same for most [grain spawn](#grain-spawn) but the most accessible source of grain spawn is `wild bird seed`.
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You should, however, provide mycelium a variety of [grain spawn and substrate](#grain-spawn-and-substrate) between generations of colonies so the strain does not lose it's ability to digest different sources of nutrition.
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These general instructions are the same for most [grain spawn](#grain-spawn-and-substrate) but the most accessible source of grain spawn is `wild bird seed`.
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# Grain Spawn
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## Grain Spawn and Substrate
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Nutrient rich grain treated and sanitized to feed mycelium.
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Vitamin and mineral rich substrated treated and sanitized to provide mycelium a growing surface.
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- [ ] 16 oz wide mouth mason jars with lids
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- [ ] wild bird seed with millet
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- [ ] colander
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- [ ] coconut coir
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- [ ] 8 liter, approximate, bucket and lid
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- [ ] aluminum foil
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- [ ] electric pressure cooker, stove top, or [autoclave](#autoclave)
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Wild bird seed is dusty and full of bacteria and molds.
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Because the seeds are dry the bacteria has likely [endosporulated](https://en.wikipedia.org/wiki/Endospore), making it resistant to sanitization.
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Soaking the bird seed will clean it up a bit and encourage the endospores to reproduce, leaving them weaker to [autoclave](#autoclave).
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Imperial | Metric
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-------- | ------
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16 oz | 450 ml
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1 cup | 250 ml
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Wild bird seed is dusty and full of bacteria and molds.
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Because the seeds are dry the bacteria has likely [endosporulated](https://en.wikipedia.org/wiki/Endospore), making it resistant to sanitization.
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Soaking the bird seed will clean it up a bit and encourage the endospores to reproduce, leaving them weaker to [autoclave](#autoclave).
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Earlier it was mentioned that mycelium want a variety of [grain spawn](#grain-spawn-and-substrate) and [substrates](https://en.wikipedia.org/wiki/Substrate_(biology)) between generations of colonies so the strain does not lose it's ability to digest different sources of nutrition.
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This guide uses coconut husk (coir) mixed with [grain spawn](#grain-spawn-and-substrate) for colonization as a substrate.
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You can also add coffee grounds, sawdust, straw, and rice husks for variety.
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Take caution to only change one thing at a time between generations or you risk overwhelming the mycelium.
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Be mindful of commercial gardening coir as they are treated with [endophyte](https://en.wikipedia.org/wiki/Endophyte) to protect plant roots.
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These endophytes do not protect our mushroom roots and will compete for resources like any other contamination.
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0. [Sanitize Your Workspace](#sanitize-your-workspace).
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1. Fill 16 oz wide mouth mason jars with 1 cup of bird seed for each final jar of grain spawn.
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0. Hydrate the coconut coir brick following the packaging instructions.
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2. Cover bird seed with tap water, lid jar, and soak for 15 minutes.
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0. Mix the coconut coir and wild bird seed in the bucket at a 50/50 ratio.
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3. Strain the bird seed with a colander and rinse with water.
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0. Fill with water until moisture is visible on the surface of the mixture and soak overnight.
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- Pick out any unwanted bits like twigs or mush.
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0. Split evenly to each 16 oz wide mouth mason jar, about 1 cup.
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- 1 hour to dry, stirring occasionally to expose moisture.
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0. Cover jar opening with aluminum foil and [pressure cook](#autoclave) for 2 hours.
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4. Split evenly to each 16 oz wide mouth mason jar, about 1 cup.
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0. Lightly screw lid over the foil to secure it in place without sealing the jar and allow to cool to room temperature.
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5. Cover jar opening with aluminum foil and [pressure cook](#autoclave) for 2 hours.
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0. Store in a dark cool place until inoculation.
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6. Lightly screw lid over the foil to secure it in place without sealing the jar and allow to cool to room temperature.
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7. Store in a dark cool place until inoculation.
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# Spawn Inoculation
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## Spawn Inoculation
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- [ ] 50 to 100 ml syringe with hollow needle, [autoclavable](#autoclave)
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0. [Sanitize Your Workspace](#sanitize-your-workspace).
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- Sanitize the outside of each jar of [grain spawn](#grain-spawn) and [liquid culture](#liquid-cultures).
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- Sanitize the outside of each jar of [grain spawn](#grain-spawn-and-substrate) and [liquid culture](#liquid-cultures).
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1. With a sanitary syringe extract 1 ml of [liquid culture](#liquid-cultures) and replace the screw top.
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1. Gently stir the [liquid culture](#liquid-cultures) and with a sanitary syringe extract 1 ml of [liquid culture](#liquid-cultures), replace the screw top.
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2. Inject syringe into the jar of [grain spawn](#grain-spawn) by carefully piercing the aluminum foil straight down along the inside of the jar.
|
||||
2. Inject syringe into the jar of [grain spawn](#grain-spawn-and-substrate) by carefully piercing the aluminum foil straight down along the inside of the jar.
|
||||
|
||||
3. Replace the mason jar lid by flipping it so the seal is facing up and lightly screwing on the rim.
|
||||
|
||||
4. Store in ambient room light at room temperature.
|
||||
|
||||
# Reading a Spawn Jar
|
||||
## Reading a Spawn Jar
|
||||
|
||||
Similar to [reading an agar slant](#reading-an-agar-slant), [grain spawn](#grain-spawn) should show growth within the first week after inoculation and should be monitored for contamination weekly until it is fully colonized, 2-3 weeks.
|
||||
Similar to [reading liquid cultures](#reading-liquid-cultures), [grain spawn](#grain-spawn-and-substrate) should show growth within the first week after inoculation and should be monitored for contamination weekly until it is fully colonized, 2-3 weeks.
|
||||
|
||||
Mycelium are snowy white with grey and blue tints as normal.
|
||||
Mycelium are snowy white with grey, blue, or brown tints depending on the species.
|
||||
|
||||
Any other colors and you are dealing with contamination.
|
||||
Colors like yellow, red, green, and black usually indicate contamination.
|
||||
Minor contamination is not the end of the world, many colonies can recover.
|
||||
|
||||
If the jar is fully colonized following a contamination and doesn't smell like rotten apples it can still produce mushrooms.
|
||||
|
||||
Once the grain in the jar is covered with feathery white mycelium, give it one more week before moving the cakes to [bulk substrate](#bulk-substrate).
|
||||
Once the grain and substrate in the jar is covered with feathery white mycelium, give it one more week before [fruiting](#fruiting).
|
||||
|
||||
# Fruiting
|
||||
|
||||
Getting mycelium to fruit is a labor intensive process.
|
||||
Fruiting isn’t necessary for the production of organic compounds.
|
||||
You could use food grade grain like steel cut oats or brown rice in [colonization](#colonization) then skip the fruiting stage by waiting until it is fully colonized and dehydrate the mycelium.
|
||||
|
||||
It is much more appetizing to prepare and consume the fruiting bodies.
|
||||
|
||||
If you've ever been walking in the woods after a light rain you've probably seen mushrooms peaking up.
|
||||
This is because mycelium sense that water is evaporating and wish to take advantage of the updraft to spread their spores.
|
||||
|
||||
In our fruiting containers we want to mimic that as much as possible with a spray bottle and a light breeze.
|
||||
In our fruiting containers we want to mimic that as much as possible without exposing the substrate to contamination.
|
||||
|
||||
This can be accomplished by opening a fully colonized [grain spawn](#grain-spawn) jar and misting the mycelium cake with water daily.
|
||||
The mushrooms are light sensative, to prevent them grow inside the jar we cover the glass with aluminum foil.
|
||||
|
||||
However, exposing more surface area give the mushrooms more room to sprout.
|
||||
|
||||
# Bulk Substrate
|
||||
|
||||
- [ ] coconut coir
|
||||
|
||||
- [ ] take-out containers, black opaque, polypropylene
|
||||
|
||||
Earlier it was mentioned that mycelium want a variety of [grain spawn](#grain-spawn) and [substrates](https://en.wikipedia.org/wiki/Substrate_(biology)) between generations of colonies so the strain does not lose it's ability to digest different sources of nutrition.
|
||||
|
||||
Once you have practice making [grain spawn](#grain-spawn) you'll want to mix 25% of the substrate chosen for this step to give the mycelium time to produce the right enzymes during colonization.
|
||||
|
||||
This guide uses coconut husk (coir) mixed with [grain spawn](#grain-spawn) for colonization as a substrate.
|
||||
You can also add coffee grounds, sawdust, straw, and rice husks for variety.
|
||||
Take caution to only change one thing at a time between generations or you risk overwhelming the mycelium.
|
||||
|
||||
Many commercial gardening coir are treated with [endophyte](https://en.wikipedia.org/wiki/Endophyte) to protect plant roots.
|
||||
These endophytes do not protect our mushroom roots and will compete for resources like any other contamination.
|
||||
|
||||
**Coconut coir must be [autoclaved](#autoclave) prior to use.**
|
||||
|
||||
Take-out containers are available at most grocery or box retailers.
|
||||
They must have black opaque bottoms with clear lids, usually made from polypropylene.
|
||||
You can tell if they are polypropylene when they are advertised as dishwasher and microwave safe.
|
||||
Polypropylene products also carry a recycling #5 designation.
|
||||
|
||||
0. Soak the coconut coir brick for 15 minutes or following the packaging instructions.
|
||||
|
||||
1. Fill mason jars with the hydrated coconut coir and [pressure cook](#autoclave) for 1 hour and cool to room temperature.
|
||||
|
||||
2. Layer the bottom of the take-out containers with 2 cm of coconut coir.
|
||||
|
||||
3. Open and invert a fully colonized [grain spawn](#grain-spawn) jar and firmly but gently tap the mycelium cake out into the open take-out container.
|
||||
Gently break up the cake with a sanitary utensil until the mycelium covers the layer of coir.
|
||||
|
||||
4. Layer the top of the mycelium layer with 2 cm of coconut coir.
|
||||
|
||||
5. Return the lid to the take-out container and store in ambient room light at room temperature.
|
||||
|
||||
# Fruiting Chambers
|
||||
## Fruiting Chambers
|
||||
|
||||
- [ ] clear plastic tubs with lids, shoebox or larger
|
||||
|
||||
- [ ] hygrometers
|
||||
|
||||
Check the mycelium in the take-out containers once a week for contamination.
|
||||
Once the top surface of the coconut coir has been covered by feathery white mycelium it is ready to fruit.
|
||||
|
||||
0. Remove lid from take-out container and place within a clear plastic tub.
|
||||
0. Cover the glass mason jars with a sheet of aluminum foil, remove lid exposing the colony to the air.
|
||||
|
||||
1. Place a sanitary hygrometer onto the tub where it is visible.
|
||||
0. Place jar within a clear plastic tub with the jar on its side.
|
||||
|
||||
2. Replace tub lid and store in ambient room light at room temperature.
|
||||
0. Place a sanitary hygrometer onto the tub where it is visible.
|
||||
|
||||
0. Once tub is full of sideways open jars, replace tub lid and store in ambient room light at room temperature.
|
||||
|
||||
## Daily
|
||||
|
||||
|
|
@ -519,8 +412,6 @@ It is necessary to open at least daily to provide fresh air and to clear out the
|
|||
|
||||
0. If needed, gently mist the mycelium cake and surrounding coconut coir a spray bottle of water until humid.
|
||||
|
||||
1. Fan the tub with the lid for a few moments then replace the tub lid.
|
||||
|
||||
Each mushroom is different generally harvest mushrooms when their gills are exposed but before they dump their spores and make a mess.
|
||||
|
||||
# Checklist
|
||||
|
|
@ -543,24 +434,18 @@ Each mushroom is different generally harvest mushrooms when their gills are expo
|
|||
|
||||
- [ ] light corn syrup
|
||||
|
||||
- [ ] diammonium phosphate with urea
|
||||
|
||||
- [ ] agar
|
||||
|
||||
- [ ] 30 to 50 ml test tubes with screw cap, [autoclavable](#autoclave)
|
||||
- [ ] marbles or magnetic stirring rods
|
||||
|
||||
- [ ] flame or lighter
|
||||
|
||||
- [ ] wild bird seed with millet
|
||||
|
||||
- [ ] colander
|
||||
- [ ] 8 liter, approximate, bucket and lid
|
||||
|
||||
- [ ] aluminum foil
|
||||
|
||||
- [ ] coconut coir
|
||||
|
||||
- [ ] take-out containers, black opaque, polypropylene
|
||||
|
||||
- [ ] clear plastic tubs with lids, shoebox or larger
|
||||
|
||||
- [ ] hygrometers
|
||||
|
|
|
|||
Loading…
Reference in New Issue