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JoYo 84f18e3662 minor 2020-02-12 21:17:59 -05:00
JoYo c6eb9120c1 minor 2020-02-12 21:09:57 -05:00
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@ -8,10 +8,10 @@ You don't have to follow every step in this guide but you should because it's fu
# Always a Risk of Contamination # Always a Risk of Contamination
Contamination occurs when other microorganisms take advantage of the gratuity you provide for your mycelium. Contamination occurs when other microorganisms take advantage of the gratuity you provide for your mycelium.
There is always a risk of bacteria and mold contamination when it comes to mycelium. There is always a risk that bacteria and mold will invade, competing with your precious mushroom makers.
Many guides do everything they can to reduce this risk. Many guides do everything they can to reduce this risk.
My approach is to give you methods that are still effective but easier to recover from contaminated spawn. My approach gives you methods that are still effective but easy and cheap to recover from contaminated spawn.
# Do It Thrice # Do It Thrice
@ -24,13 +24,13 @@ As a result of the above hubris we triple our efforts.
- Make one extra of the above as a control for testing your sanitation. - Make one extra of the above as a control for testing your sanitation.
It is OK. It is OK.
Seed spawn, agar, and sugar water are inexpensive. Bird seed, agar powder, and sugar water are inexpensive.
All other equipment can be reused. All other equipment can be reused.
Saving a [control](https://en.wikipedia.org/wiki/Scientific_control) will help you test for contamination introduced through your technique. Saving a [control](https://en.wikipedia.org/wiki/Scientific_control) will help you test for contamination introduced through your technique.
If all fares well you can always end up using it. If all fares well you can always end up using it.
Spawn medium refers to any growing surface, such as grain spawn or culture mediums. Spawn medium refers to any nutrient rich growing surface, such as grain spawn or culture mediums.
# Finding Samples # Finding Samples
@ -41,10 +41,10 @@ The end of this document has a combined checklist.
Newbies should start with grocery store mushrooms. Newbies should start with grocery store mushrooms.
This will allow you to walk through the methods without fear of wasting a sample. This will allow you to walk through the methods without fear of wasting a sample.
Getting other samples should not be difficult but it is quite frustrating to discover all the precautions were made in vain when a contaminated sample was the source. Finding other kinds of mycelium samples is not particularly difficult but it is frustrating to discover all the sanitary precautions were made in vain when a contaminated sample was the source.
Of course there is always the risk of contaminated grocery store mushrooms. There remains the risk that grocery store mushrooms are contaminated.
Online shopping can get you all sorts of sample types ranging from spawn grains and liquid culture to spores and dried mushrooms. Online shopping can get you all sorts of mycelium sample types, ranging from spawn grains and liquid culture to spores and dried mushrooms.
When paying good money for a sample try to find liquid culture as they have a pretty good colonization rate. When paying good money for a sample try to find liquid culture as they have a pretty good colonization rate.
Eventually you will want to make your own cultures from your harvested mushrooms. Eventually you will want to make your own cultures from your harvested mushrooms.
@ -54,6 +54,8 @@ Eventually you will want to make your own cultures from your harvested mushrooms
- [ ] empty spray bottles, chemical resistant - [ ] empty spray bottles, chemical resistant
- [ ] star san, sanitizer - [ ] star san, sanitizer
The critical time to practice good sanitation is when opening any of your containers and interacting with the spawn medium inside.
Clean yourself and work area before working with a mycelium sample or spawn medium. Clean yourself and work area before working with a mycelium sample or spawn medium.
All surfaces and tools should be wiped clean then sprayed with a sanitizing solution to soak for at least 2 minute prior to working. All surfaces and tools should be wiped clean then sprayed with a sanitizing solution to soak for at least 2 minute prior to working.