dap and malt extract for the homebrewers

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JoYo 2020-02-15 16:44:22 -05:00
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@ -52,6 +52,7 @@ Eventually you will want to make your own cultures from your harvested mushrooms
# Sanitize Your Workspace
- [ ] empty spray bottles, chemical resistant
- [ ] star san, sanitizer
The critical time to practice good sanitation is when opening any of your containers and interacting with the spawn medium inside.
@ -126,9 +127,13 @@ Skipping testing on [agar slants](#agar-slants) works well enough as long as you
# Culture Mediums
- [ ] food scale, grams
- [ ] distilled water
- [ ] 16 oz wide mouth mason jars with lids
- [ ] electric pressure cooker, stove top, or [autoclave](#autoclave)
- [ ] aluminum foil
imperial | metric
@ -138,6 +143,7 @@ imperial | metric
## Liquid Cultures
- [ ] light corn syrup
- [ ] diammonium phosphate with urea (optional)
Mixing liquid culture is similar to [agar slants](#agar-slants) without needing to cool the test tubes in any particular position.
A simple sugar like light corn syrup is dissolved in distilled water to provide mycelium calories for reproduction.
@ -157,6 +163,8 @@ simple sugar | 15 g | 5 g
3. Add 15 grams of light corn syrup to warm water and stir for 1 minute with a sanitary utensil.
- Optionally add no more than 1 gram of diammonium phosphate (dap) with urea for yeast nutrients.
4. Cover un-lid jar opening with aluminum foil and [autoclave](#autoclave) for 1 hour.
5. Allow to cool and replace aluminum foil with mason jar lid.
@ -168,20 +176,23 @@ simple sugar | 15 g | 5 g
## Agar Slants
- [ ] malt extract agar
- [ ] 30 to 50 ml test tube with screw cap, [autoclavable](#autoclave)
- [ ] 50 to 100 ml syringe with hollow needle, [autoclavable](#autoclave)
Agar agar is a plant gelatin that solidifies at room temperature, providing a physical surface that mycelium to colonize.
This is mixed with a simple sugar like malt extract to provide mycelium calories for reproduction.
When mixed with a simple sugar like malt extract this provides mycelium calories for reproduction.
Malt Extract Agar (MEA) is available premixed fairly cheap online.
Mixing 2:3 grams ratio simple sugar to Agar powder is good for saving money in bulk.
Mixing 2:3 grams ratio of malt extract to agar powder is good for saving money by buying in bulk from a homebrew shop.
Scale the following recipe as needed to fill 1/3 of each test tube or 10 ml:
ingredient | mason jar | ratio
---------- | --------- | -----
water | 300 ml | 100 ml
MEA | 15 g | 5 g
agar | 9 g | 3 g
malt extract | 6 g | 2 g
0. [Sanitize Your Workspace](#sanitize-your-workspace).
@ -191,7 +202,7 @@ MEA | 15 g | 5 g
2. Microwave with a safe cover until boiling.
3. Add 15 grams of MEA to warm water and stir for 1 minute with a sanitary utensil.
3. Add 9 g of agar and 6 g of malt extract to the warm water and stir well for 1 minute with a sanitary utensil.
4. While the MEA solution is still warm, fill a sanitary syringe with the solution.
@ -248,6 +259,7 @@ This will allow you to walk through the methods without fear of wasting a spore
## Cloning
- [ ] paring knife or scalpel
- [ ] 50 to 100 ml syringe with hollow needle, [autoclavable](#autoclave)
Taking mycelium samples from mushroom tissue is called cloning.
@ -270,6 +282,7 @@ Piercing the mushroom tissue with a hollow needled syringe is enough to collect
## Spore Samples
- [ ] inoculation loop
- [ ] flame or lighter
[Spore](https://en.wikipedia.org/wiki/Fungus#Spore_dispersal) samples are created by placing a mushroom cap on a flat surface and collecting the spores that are released.
@ -354,11 +367,17 @@ I leave it up to you to research your mycelium species.
# Grain Spawn
- [ ] 16 oz wide mouth mason jars with lids
- [ ] wild bird seed with millet
- [ ] colander
- [ ] ladle
- [ ] boiling pot
- [ ] aluminum foil
- [ ] electric pressure cooker, stove top, or [autoclave](#autoclave)
Wild bird seed is dusty and full of bacteria and molds.
@ -439,7 +458,9 @@ However, exposing more surface area give the mushrooms more room to sprout.
# Fruiting Chambers
- [ ] medium plastic tubs with lids
- [ ] coconut coir
- [ ] personal fan
Coconut husk (coir) as casing layer and flooring in our fruiting chamber to hold moisture better than the mycelium cake we created in the [grain spawn](#grain-spawn) jar.
@ -496,6 +517,8 @@ Try to maintain 90% humidity within the fruiting chamber.
- [ ] light corn syrup
- [ ] diammonium phosphate with urea
- [ ] paring knife or scalpel
- [ ] inoculation loop